Cau truc mang

Consider transfer of 2 electrons from NADH to oxygen:
a. ½ O
2
+ 2H
+
+ 2e
-
 H
2
O
E°' = +0.815 V
b. NAD
+
+ 2H
+
+ 2e
-
 NADH + H
+
E°' = −0.315 V
Subtracting reaction b from a:
c. ½ O
2
+ NADH + H
+
 H
2
O + NAD
+
∆E°'= +1.13 V
∆G = − nF∆E
o
' = – 2(96494)(1.13) = – 218 kJ/mol

Electron Carriers
NAD
+
/NADH and FAD/FADH
2
were introduced
earlier.
FMN (Flavin MonoNucleotide) is a prosthetic group
of some flavoproteins.
It is similar in structure to FAD (Flavin Adenine
Dinucleotide), but lacking the adenine nucleotide.
FMN (like FAD) can accept 2 e
-
+ 2 H
+
to form
FMNH
2
.

FMN, when bound at the active site of some enzymes, can
accept 1 e
−
to form the half-reduced semiquinone radical.
The semiquinone can accept a 2
nd
e
−
to yield FMNH
2
.
Since it can accept/donate 1 or 2 e
−
, FMN has an important
role mediating e
−
transfer between carriers that transfer 2e
−

(e.g., NADH) & those that can accept only 1e
−
(e.g., Fe
+++
).

C
C
C
H
C
C
H
C
N
C
C
N
N
C
NH
C
H
3
C
H
3
C
O
O
CH
2
HC
HC
HC
H
2
C
OH
O P O-
O
O-
OH
OH
C
C
C
H
C
C
H
C
N
C
C
H
N
N
C
NH
C
H
3
C
H
3
C
O
O
CH
2
HC
HC
HC
H
2
C
OH
O P O-
O
O-
OH
OH
C
C
C
H
C
C
H
C
N
C
C
H
N
N
H
C
NH
C
H
3
C
H
3
C
O
O
CH
2
HC
HC
HC
H
2
C
OH
O P O-
O
O-
OH
OH
e
−

+

H
+
e
−

+

H
+

FMN
FMNH
2

FMNH
·


Coenzyme Q (CoQ, Q, ubiquinone) is very hydrophobic.
It dissolves in the hydrocarbon core of a membrane.
It includes a long isoprenoid tail, with multiple units having
a carbon skeleton comparable to that of isoprene.
In human cells, most often n = 10.
Q
10
’s isoprenoid tail is longer than the width of a bilayer.
It may be folded to yield a more compact structure, & is
postulated to reside in the central domain of a membrane,
between the 2 lipid monolayers.

O
O
CH
3
O
CH
3
CH
3
O
(CH
2
CH C CH
2
)
n
H
CH
3
coenzyme Q






isoprene
H
2
C
C C
CH
2
CH
3
H

The quinone ring of
coenzyme Q can be
reduced to the quinol
in a 2e
−
reaction:

O
O
CH
3
O
CH
3
CH
3
O
(CH
2
CH C CH
2
)
n
H
CH
3
OH
OH
CH
3
O
CH
3
CH
3
O
(CH
2
CH C CH
2
)
n
H
CH
3
2

e
−
+ 2

H
+

coenzyme Q
coenzyme QH
2

Q + 2 e
−
+ 2 H
+
 QH
2
.

When bound to special sites in respiratory complexes, CoQ
can accept 1 e
−
to form a semiquinone radical (Q·
−
).
Thus CoQ, like FMN, can mediate between 1 e
−
& 2 e
−

donors/acceptors.

O
O
CH
3
O
CH
3
CH
3
O
(CH
2
CH C CH
2
)
n
H
CH
3
OH
OH
CH
3
O
CH
3
CH
3
O
(CH
2
CH C CH
2
)
n
H
CH
3
e
−
+ 2

H
+

coenzyme Q
coenzyme QH
2

O
−
O
CH
3
O
CH
3
CH
3
O
(CH
2
CH C CH
2
)
n
H
CH
3
e
−

coenzyme Q

•
−


Coenzyme Q functions as a mobile e
−
carrier within
the mitochondrial inner membrane.
Its role in trans-membrane H
+
transport coupled to e
−

transfer (Q Cycle) will be discussed later.

Heme is a prosthetic group of cytochromes.
Heme contains an iron atom in a porphyrin ring system.
The Fe is bonded to 4 N atoms of the porphyrin ring.

N
N
N
N
CH
3
HC
CH
3
S CH
2
CH
3
CH S CH
2
CH
3
CH
2
CH
2
COO
−
CH
3
H
3
C
CH
2
CH
2
−
OOC
protein
protein
Fe
Heme c


Hemes in the 3 classes of cytochrome (a, b, c) differ slightly in
substituents on the porphyrin ring system. A common feature is
2 propionate side-chains. Only heme c is covalently linked to
the protein via thioether bonds to cysteine residues.

N
N
N
N
CH
3
HC
CH
3
S CH
2
CH
3
CH S CH
2
CH
3
CH
2
CH
2
COO
−
CH
3
H
3
C
CH
2
CH
2
−
OOC
protein
protein
Fe
Heme c

Heme a is unique in having a long farnesyl side-chain
that includes 3 isoprenoid units.

N
N
N
N
CH
3
HC
CH
2
CH
3
CH CH
2
CH
2
CH
2
COO
−
CH
3
HC
CH
2
CH
2
−
OOC
Fe
OH
CH
2
CH C CH
2
CH
3
3
H
O
Heme a

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